CRISPR diversifies: Cut, paste, on, off, and now—evolve
Innovative Genomics Institute
Natural DNA variation is akin to this process – as time goes by, random changes pop up across the genomes of different individual organisms. Step-by-step, how EvolvR targets a specific sequence for diversification. The researchers are especially excited about combining EvolvR with another CRISPR toolbox favorite, high-throughput CRISPR screening. This potent pairing of technologies would let them diversify thousands of different genes in a single experiment, potentially creating brand new functions instead of just turning genes on and off. A single drop of bacteria diversified with EvolvR contains immense diversity.